Attenuation of Transforming Growth Factor-? Expression by Alteplase in Anterior Lens Capsule Fibrosis Model with Fibrin Reaction In Vitro

Background: Underlying pathophysiology of posterior capsule opacification, a frequent visual disturbance postop complication cataract surgery, is multifactorial. Several cytokines and growth factors, such as Transforming Growth Factor-? (TGF-?), Fibroblast Factor 2 (FGF2), Hepatocyte (HGF), have been found to play roles in the formation PCO. Multiple advancements made including researches therapeutic agents. Alteplase, fibrinolytic agent, has studied decrease fibrin formation, which mechanism causing This study aimed investigate effect alteplase on TGF-? expression an anterior lens fibrosis model resembling Methods: An experimental was performed using cultured tissue taken during surgery. Primary cell isolation culture epithelial characterization were conducted. Following identification 90% confluency rate culture, cells divided into four groups then scratched induce inflammatory reaction PCO process after Doses 25 mcg/ml, 50 100 mcg/ml applied each group cultures with other serving negative control. Using fluorescein microscope, measurements asses influence 7 days scratching by analyzing intensity fluoresce FITC antibody TGF-?. Results: The attenuation observed all treatment groups; µg/ml (15.5716x106 ±2.56558x106 ), (12.7364x106 ±1.67067x10 6 (7.8422x106 ±2.24941x106). Tukey HSD posthoc tests significant between control (p=0.019, p=0.000, p=0.000; respectively). Conclusion: Alteplase within measure dose range holds potential inhibiting through